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Work with membrane proteins without using cell cultures

With Ciloa, you no longer have to use cell cultures to work with membrane proteins, no matter what you are working on:

 

Membrane proteins that are easy to handle

Now you have an easy way to handle native membrane proteins in suspension, in homogenous, characterized and reliable batches. Ciloa enables you to go farther with your dreams, like you've never been able to do before.

  ciloa exosome electron microscopy 2

Fully native membrane proteins on exosomes

Use fully native and well-folded membrane proteins on natural nanovesicles - exosomes - for your ligand screening and monoclonal antibody development.
Leverage Ciloa to imagine new kinds of vaccines, therapeutics, and diagnostics.

Developing therapeutic antibodies

Fully native transmembrane antigens are produced, in the purest available form, on natural nanovesicles called exosomes, known to be potent immuno-stimulators. Now ciloa offers you a tool to develop monoclonal antibodies and immune responses against single and multi-transmembrane proteins (GPCR, receptor tyrosine kinase, adhesion molecules, viral envelope proteins ...).

Screening ligand binding to membrane receptors

Membrane receptors are sorted in their native conformation at high concentrations on cell-secreted nanovesicles, or exosomes. Characterized and homogenous receptor batches can be prepared, generating reliable screening of both endogenous and exogenous ligands using FRET, SPR, and cytofluorometric methods.

Developing new diagnostics

Producing native transmembrane proteins on exosomes makes it possible to develop new kinds of diagnostic tests to detect molecules that are specific to the conformational structure of membrane proteins.  Several tags, such as biotin, GFP, hexa-His, and more, can be added on the targeted membrane proteins to help detection.  Detection can be performed using classic ELISA techniques or SPR.

Meet Ciloa

ciloa-conference
Meet Ciloa at the BIO International Convention June 18-21th, 2012 in Boston, MA.Let’s meet !

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