Fully native transmembrane antigens are produced, in the purest available form, on natural nanovesicles called
exosomes, known to be potent
immuno-stimulators. Now ciloa offers you a tool to develop
monoclonal antibodies and immune responses against single and multi-transmembrane proteins (GPCR, receptor tyrosine kinase, adhesion molecules, viral envelope proteins ...).
Membrane receptors are sorted in their native conformation at high concentrations on cell-secreted nanovesicles, or
exosomes. Characterized and homogenous receptor batches can be prepared, generating reliable screening of both endogenous and exogenous ligands using FRET, SPR, and cytofluorometric methods.
Producing native transmembrane proteins on
exosomes makes it possible to develop new kinds of
diagnostic tests to detect molecules that are specific to the conformational structure of membrane proteins. Several tags, such as biotin, GFP, hexa-His, and more, can be added on the targeted membrane proteins to help detection. Detection can be performed using classic ELISA techniques or SPR.