Monoclonal antibodies

Generally speaking, it is a challenge to develop monoclonal antibodies recognizing conformational epitopes. This is particularly true for proteins with multi-transmembrane domains, such as GPCR.

Ciloa offers clients the ability to leverage unique qualities of membrane proteins harbored by exosomes, thus helping at several stages:

1. Immunization

The development of specific immune response is helped by:

• Exosome functions in natural immune response
• High concentration of antigen
• Few contaminants
• Proteins with fully native conformation

2. Screening of hybridomas

Rapid selection of monoclonal antibody hits by ELISA, SPR (Surface Plasmon Resonance) and/or by cytofluorimetry:

• No antigen labeling required
• Screening automation
• Immediate access to affinity constants

3. Affinity enhancement

Easier affinity enhancement of leads using phage-display:

• Baits with few contaminants
• Easy-to-handle baits
• Baits that can be coated on supports