ANY TYPE OF MEMBRANE PROTEINS WITH THEIR FULLY NATIVE CONFORMATION

Ciloa’s unique technology makes possible to produce any type of membrane proteins (GPCR, kinase receptor, ion channel, viral protein, etc.) with their fully native conformation on exosomes outside the cell. It allows to load exosomes with cytosolic proteins as well.

It is based on the discovery of an exosomal pilot peptide that contains motifs that interact with so-called ESCRT proteins.

The fusion of the exosomal pilot peptide with a membrane protein triggers its active sorting and secretion with exosomes. Ciloa successfully beneficiates of two natural cellular pathways :

  • The ER/Golgi pathway that allows synthesis, correct folding, oligomerization and post-translational modifications of membrane proteins. At each step, the membrane proteins are submitted to strong quality controls.
  • A specific sorting pathway involving ESCRT machinery that allows the secretion outside of the cells, of the membrane proteins imbedded into the membrane of natural nanovesicles called exosomes.

UNIQUE IN VIVO TECHNOLOGY

A UNIQUE HIGH POTENTIAL TECHNOLOGY

FULLY NATIVE MEMBRANE PROTEIN COMPLEXES OUT OF CELLS
Because it uses all-natural pathways, the Ciloa technology platform allows to produce membrane proteins with their fully native conformation in a concentrated form outside the cell. In addition, several proteins can be targeted on the same exosomes, allowing the production of exosomes harboring homo- or hetero-oligomers complexes outside the cells.
INTERNATIONAL PATENTS WITH FULL FREEDOM TO OPERATE
The core of Ciloa technology has been developed in an academic laboratory. The 2 family patents originated are owned by the CNRS and the University of Montpellier. Ciloa has the worldwide exclusive licence on these fully delivered patents (with full freedom to operate). These patents are strengthened by 8 years of know-how.
MORE THAN 80 PROTEINS INCLUDING 60 MEMBRANE PROTEINS
Ciloa has produced more than 60 membrane proteins of all types (GPCR, kinase receptors, transporters, ion channels, viral membrane proteins …) on natural nanovesicles (exosomes). Of them, are 28 GPCRs, viral envelope protein multimers and hetero oligomers of ion channel complexes.
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GPCRs
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Kinase receptors
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Ion channels
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Viral Proteins
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